Nanospray for LTQ
It is now possible to take full advantage of easy, repeatable nano spray using the the Agilent Chip product with Thermo LTQ, Velos, Orbitrap, Q Exactive or Vantage triple quadrupole mass spectrometers. Our own CSS Analytical TrueSpray(c) Interface or an Agilent HPLC-Chip Cube MS Interface (G4240A) with our custom built interface easily attaches to any Thermo Scientific mass spectrometer with an IonMax (or newer) source housing.
The Agilent Chip (various part numbers) is a credit card sized, disposable product, which offers an enrichment column, an analytical column, a column switching valve and an electrospray (ESI) emitter – all on one micro-fabricated chip.
CSS Analytical modifies both the Agilent Chip Cube Box and the individual Chips. We sell both new and used pre-modified Chip Cube Boxes and also modified Chips for very reasonable prices. We service and support our modified cube boxes and in some cases will even warranty your Agilent Box if modified by us. We can also arange for short term rental or long term lease of the Agilnet hardware. Contact us for details.
This two-column chip is the key, it provides easy, repeatable, walk up – drop and spray nano ESI. Set up time is virtually non-existent. If your lab needs nano spray for its own obvious benefits on your LTQ then the CSS Analytical Nanospray Interface system is what you need. Click here to fill out our easy quotation form.
The system configuration looks like this: There are two HPLC pumping systems, which can be any brand but we think that Agilent 1100 and 1200 systems are awesome. A Micro-capillary pumping system will load the On-Chip trapping column. After the trapping column is loaded, the On-Chip valve switches and a Nano pumping system takes over to elute the analyte off the trapping column and onto the On-Chip analytical column. Loading is done at around 4.0 ul/min, the analytical gradient is run at 500 nl/min. Of course a PC and Chemstation will be required to run the Agilent modules. We can also help you connect your other HPLC systems, any brand and any software. We have been very resourceful in setting up the PC system for joint LTQ, Chip Delivery System and HPLC control. All parts of the system are interconnected with remote start cables so full sequencing is straight forward, making automated runs easy.
CSS Analytical Co Inc is not affiliated with Agilent Technologies in this project and product offerings. Agilent Technologies is not in any way connected with the CSS Analytical Chip Delivery System and does not sanction or warrant any modifications to its products.
Rapid Protein ID Chip: 43mm 300A C18 Analytical Column, 40 nl Trapping Column
Chip in spray mode, note the nano-spray bending slightly to the left
Chip in spray mode, note the nano-spray bending slightly to the left
Agilent Chip Cube on Thermo LTQ via CSS Analytical Interface hardware
Here is our Chip Delivery System (CDS) attached to a Thermo LTQ Velos with Orbitrap
LTQ in our own lab, with the CDS. We're up and running daily working on application notes
Here's a chromatograph of Digested IGG, eluted with a 10 minute gradient from 5% Acn to 70% Acn using the Harvard Syringe pumps and our gradient control program, on the Agilent Protein ID Chip. Rapid Protein ID, 43mm 300 A C18 chip w/40 nL traping column.
Check out our 2D HPLC pumping system. Micro flow loading and Nano flow eluting. We have written a visual basic executable to program a true gradient and control the solvent delivery. Three pumps, nano ported switching valve and software for under $25k. Contact us by email for details.
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"Thermo", "LTQ" and "Velos" are trademarks of Thermo Fisher Scientific Inc.
